S100A8 antibody

Catalog No. ABIN6296169

This anti-S100A8 antibody is a Mouse Monoclonal antibody detecting S100A8 in IHC, IF and FACS. Suitable for Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey, Horse, Guinea Pig, Baboon and Goat.

Quick Overview for S100A8 antibody (ABIN6296169)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey, Horse, Guinea Pig, Baboon, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-S100A8 Antibody

Purpose: Mouse anti-Human/Baboon/Monkey/Cow/Pig/Goat/Horse/Cat/Dog/Rabbit/Guinea Pig/Rat/Mouse Macrophage Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: An affinity purified monocyte membrane preparation was used as the immunogen for the anti-Macrophage antibody.

Immunogen: An affinity purified monocyte membrane preparation was used as the immunogen for the anti-Macrophage antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: Protein S100-A8

Background:

Target Description: Recognizes the macrophage L1 protein Calprotectin, expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Gene Symbol: S100A8///CAGA///CFAG///MRP8///S100A9///CAGB///CFAG///MRP14

UniProt: P05109, P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins