Keratin 77 antibody

Catalog No. ABIN6296178

This anti-Keratin 77 antibody is a Mouse Monoclonal antibody detecting Keratin 77 in IHC, WB and IF. Suitable for Human and Rat.

Quick Overview for Keratin 77 antibody (ABIN6296178)

Target: Keratin 77 (KRT77)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Keratin 77 Antibody

Purpose: Mouse anti-Human/Rat Acidic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.

Immunogen: An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL (3)
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: Keratin, type II cytoskeletal 1b

Background:

Target Description: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

Gene Symbol: KRT77///KRT1B

Gene ID: 374454

UniProt: Q7Z794