CD86 antibody

Catalog No. ABIN6296200

This Mouse Monoclonal antibody specifically detects CD86 in FACS, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD86 antibody (ABIN6296200)

Target: CD86

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD86 Antibody

Purpose: Mouse anti-Human/Mouse/Rat CD86 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, membrane

Purification: ARH-77 (B-lymphoblastoid cell line) was used as the immunogen for this CD86 antibody.

Immunogen: ARH-77 (B-lymphoblastoid cell line) was used as the immunogen for this CD86 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD86

Target: CD86

Alternative Name: T-lymphocyte activation antigen CD86

Background:

Target Description: This antibody recognizes a protein of 70 kDa, which is identified as CD86 (HLDA V, WS Code BP BP072. HLDA V, WS Code A A109. HLDA VI, WS Code BP 95. HLDA VI, WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by Bcell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in Tcell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Gene Symbol: CD86///CD28LG2

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation