Cyclin A antibody

Catalog No. ABIN6296265

This anti-Cyclin A antibody is a Mouse Monoclonal antibody detecting Cyclin A in IF and FACS. Suitable for Human, Mouse, Cow, Green Monkey and Mink.

Quick Overview for Cyclin A antibody (ABIN6296265)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human, Mouse, Cow, Green Monkey, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cyclin A Antibody

Purpose: Mouse anti-Human/Mouse/Cow/African Green Monkey/Mink (Mustela) Cyclin A2 Antibody [Sodium Azide Free]

Specificity: Predominantly nuclear

Purification: Full length bovine protein was used as the immunogen for this CCNA2 antibody.

Immunogen: Full length bovine protein was used as the immunogen for this CCNA2 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL,

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin-A2

Background:

Target Description: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk 's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk 's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Gene Symbol: CCNA2///CCN1///CCNA

Gene ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA