RNF40 antibody (C-Term)

Catalog No. ABIN629630

The Rabbit Polyclonal anti-RNF40 antibody has been validated for WB and IHC. It is suitable to detect RNF40 in samples from Human, Mouse, Rat and Dog.

Quick Overview for RNF40 antibody (C-Term) (ABIN629630)

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RNF40 Antibody

Binding Specificity: C-Term

Specificity: RNF40 antibody was raised against the C terminal of RNF40

Purification: Purified

Immunogen: RNF40 antibody was raised using the C terminal of RNF40 corresponding to a region with amino acids KLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELT

Application Details

Application Notes: WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

RNF40 Blocking Peptide, (ABIN939906), is also available for use as a blocking control in assays to test for specificity of this RNF40 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of RNF40 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for RNF40

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Alternative Name: RNF40

Background: RNF40 contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.

Molecular Weight: 114 kDa (MW of target protein)