Keratin 77 antibody

Catalog No. ABIN6296409

This anti-Keratin 77 antibody is a Mouse Monoclonal antibody detecting Keratin 77 in IHC, WB, IF and FACS. Suitable for Human, Rat, Cow, Dog, Mouse, Rabbit, Chicken and Monkey.

Quick Overview for Keratin 77 antibody (ABIN6296409)

Target: Keratin 77 (KRT77)

Reactivity: Human, Rat, Cow, Dog, Mouse, Rabbit, Chicken, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Keratin 77 Antibody

Purpose: Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken pan Cytokeratin Antibody Cocktail [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Immunogen: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: Keratin, type II cytoskeletal 1b

Background:

Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Gene Symbol: KRT77///KRT1B///KRT76///KRT2B///KRT2P

UniProt: Q7Z794, Q01546