Major Capsid Protein L1 antibody
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- Target
- Major Capsid Protein L1
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Reactivity
- Human Papillomavirus 16 (HPV-16)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- Mouse anti-Type 16 of Human Papilloma Virus (HPV-16) L1 Antibody [Sodium Azide Free]
- Specificity
- Nuclear
- Purification
- Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.
- Immunogen
- Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.
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- Application Notes
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Western blot: 1-2 μg/mL,ELISA: assay dependent
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1) - Restrictions
- For Research Use only
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- Buffer
- In 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Target
- Major Capsid Protein L1
- Synonyms
- major capsid protein L1 antibody, L1 antibody
- Target Type
- Viral Protein
- Background
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Target Description: Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
Gene Symbol: L1
- UniProt
- P03101
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