CEACAM5 antibody

Catalog No. ABIN6296534

The Mouse Monoclonal anti-CEACAM5 antibody has been validated for FACS, IF, IHC, ELISA and Coat. It is suitable to detect CEACAM5 in samples from Human and Monkey.

Quick Overview for CEACAM5 antibody (ABIN6296534)

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Coating (Coat)

Product Details anti-CEACAM5 Antibody

Purpose: Mouse anti-Human/Monkey CEA Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and luminal surface

Purification: Human recombinant CEA protein was used as the immunogen for this antibody.

Immunogen: Human recombinant CEA protein was used as the immunogen for this antibody.

Application Details

Application Notes: ELISA: order BSA-free format for coating (2)
FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (3)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CEACAM5

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Alternative Name: Carcinoembryonic antigen-related cell adhesion molecule 5

Background:

Target Description: This antibody recognizes proteins of 80-200 kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Gene Symbol: CEACAM5///CEA

Gene ID: 1048

UniProt: P06731