EGFR antibody

Catalog No. ABIN6296699

The Mouse Monoclonal anti-EGFR antibody is suitable to detect EGFR in samples from Human. It has been validated for FACS, IP, AP, Func, Inhibition, in vivo, Act and ProA.

Quick Overview for EGFR antibody (ABIN6296699)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Affinity Purification (AP), Functional Studies (Func), Blocking Antibody (Inhibition), In vivo Studies (in vivo), Activation (Act), Proliferation Assay (ProA)

Product Details anti-EGFR Antibody

Purpose: Mouse anti-Human EGF Receptor Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Microsomes from A431 cells were used as the immunogen for this antibody.

Immunogen: Microsomes from A431 cells were used as the immunogen for this antibody.

Application Details

Application Notes: Functional Activity (Inhibition): antibody blocks EGF receptor activation, inhibits A431 cell proliferation, arrests tumor growth in vivo, affinity purification
IP: 1-2 μg/500 μg protein lysate
FACS: 0.5-1 μg/million cells

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: Epidermal growth factor receptor

Background:

Target Description: This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa  induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor.  < a href=../search_result.php?search_txt=egfr>EGFR  is type I receptor tyrosine kinase with sequence homology to  < a href=../search_result.php?search_txt=erbb2>ErbB2, -3 -4 or  < a href=../search_result.php?search_txt=her2>HER2, -3 -4. It binds to Epidermal Growth Factor (EGF),  < a href=../search_result.php?search_txt=TGFa>Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

Gene Symbol: EGFR///ERBB///ERBB1///HER1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins