CD99 antibody

Catalog No. ABIN6296816

The Mouse Monoclonal anti-CD99 antibody has been validated for FACS, WB, IHC and IF. It is suitable to detect CD99 in samples from Human and Rat.

Quick Overview for CD99 antibody (ABIN6296816)

Target: CD99

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD99 Antibody

Purpose: Mouse anti-Human/Rat CD99 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 5-10 µL/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD99

Target: CD99

Alternative Name: CD99 antigen

Background:

Target Description: CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]

Gene Symbol: CD99///MIC2///MIC2X///MIC2Y

Gene ID: 4267

UniProt: P14209