IGHA1 antibody

Catalog No. ABIN6296837

The Mouse Monoclonal anti-IGHA1 antibody is suitable to detect IGHA1 in samples from Human. It has been validated for IHC, FACS and IF.

Quick Overview for IGHA1 antibody (ABIN6296837)

Target: IGHA1 (Immunoglobulin Heavy Constant alpha 1 (IGHA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGHA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-IGHA1 Antibody

Purpose: Mouse anti-Human IgA Antibody [Sodium Azide Free]

Specificity: Cytoplasm, cell surface and secreted

Purification: Purified human alpha heavy chain was used as the immunogen for the Anti-IgA antibody.

Immunogen: Purified human alpha heavy chain was used as the immunogen for the Anti-IgA antibody.

Application Details

Application Notes: Flow Cytometry: 1-2 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL 30 min RT
Immunohistochemistry (FFPE): 0.5-1 μg/mL 30 min RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for IGHA1

Target: IGHA1 (Immunoglobulin Heavy Constant alpha 1 (IGHA1))

Alternative Name: Immunoglobulin heavy constant alpha 1

Background:

Target Description: This mAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Alpha heavy chain. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins. IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Gene Symbol: IGHA1///IGHA2

UniProt: P01876, P01877