Glypican 3 antibody

Catalog No. ABIN6296942

The Mouse Monoclonal anti-Glypican 3 antibody has been validated for IF, FACS and IHC. It is suitable to detect Glypican 3 in samples from Human and Rat.

Quick Overview for Glypican 3 antibody (ABIN6296942)

Target: Glypican 3 (GPC3)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-Glypican 3 Antibody

Purpose: Mouse anti-Human/Rat Glypican-3 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.

Immunogen: Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: Glypican-3

Background:

Target Description: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Gene Symbol: GPC3///OCI5

Gene ID: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process