CD34 antibody

Catalog No. ABIN6297083

The Rabbit Polyclonal anti-CD34 antibody is suitable to detect CD34 in samples from Human and Rat. It has been validated for FACS, WB, IHC and IF.

Quick Overview for CD34 antibody (ABIN6297083)

Target: CD34

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD34 Antibody

Purpose: Rabbit anti-Human/Rat Recombinant CD34 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 2-4 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD34

Target: CD34

Alternative Name: Hematopoietic progenitor cell antigen CD34

Background:

Target Description: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

Gene Symbol: CD34

Gene ID: 947

UniProt: P28906