Cytokeratin 2 antibody

Catalog No. ABIN6297226

This Mouse Monoclonal antibody specifically detects Cytokeratin 2 in WB, IHC, IF, ELISA, FACS and Coat. It exhibits reactivity toward Human and Rat.

Quick Overview for Cytokeratin 2 antibody (ABIN6297226)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Coating (Coat)

Product Details anti-Cytokeratin 2 Antibody

Purpose: Mouse anti-Human/Rat Basic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.

Immunogen: Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.

Application Details

Application Notes: ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: Keratin, type II cytoskeletal 2 oral

Background:

Target Description: This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Gene Symbol: KRT76///KRT2B///KRT2P

Gene ID: 51350

UniProt: Q01546