Recombinant Cytokeratin 2 antibody

Catalog No. ABIN6941456

The Rabbit Monoclonal anti-Cytokeratin 2 antibody (Clone KRTH-1576R) (ABIN6941456) specifically detects Cytokeratin 2 in IHC. The antibody is reactive with Human and Dog samples.

Quick Overview for Recombinant Cytokeratin 2 antibody (ABIN6941456)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: KRTH-1576R

Product Details anti-Cytokeratin 2 Antibody

Purpose: Recombinant Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody

Specificity: This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Cross-Reactivity (Details): Expected to show broad species reactivity.

Purification: 200ug/ml of recombinant MAb Purified by Protein A/G.

Immunogen: Recombinant full-length human KRT76 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: Epithelial cells, Skin or Adenocarcinomas.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: KRT76

Background:

KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Epithelial cells, Skin or Adenocarcinomas.

Molecular Weight: 52-67kDa

Gene ID: 51350, 654392

UniProt: Q01546