Cytokeratin 2 antibody

Catalog No. ABIN6297228

This anti-Cytokeratin 2 antibody is a Rabbit Polyclonal antibody detecting Cytokeratin 2 in WB, IHC, IF and FACS. Suitable for Human.

Quick Overview for Cytokeratin 2 antibody (ABIN6297228)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cytokeratin 2 Antibody

Purpose: Rabbit anti-Human Recombinant Basic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Immunogen: Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: Keratin, type II cytoskeletal 2 oral

Background:

Target Description: This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Gene Symbol: KRT76///KRT2B///KRT2P

Gene ID: 51350

UniProt: Q01546