Keratin 77 antibody

Catalog No. ABIN6297232

This Mouse Monoclonal antibody specifically detects Keratin 77 in IHC, WB, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for Keratin 77 antibody (ABIN6297232)

Target: Keratin 77 (KRT77)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Keratin 77 Antibody

Purpose: Mouse anti-Human/Rat Acidic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.

Immunogen: Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: Keratin, type II cytoskeletal 1b

Background:

Target Description: This mAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Gene Symbol: KRT77///KRT1B

Gene ID: 374454

UniProt: Q7Z794