BCL6 antibody

Catalog No. ABIN6297277

This Mouse Monoclonal antibody specifically detects BCL6 in IHC, FACS and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for BCL6 antibody (ABIN6297277)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-BCL6 Antibody

Purpose: Mouse anti-Human/Mouse Bcl6 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Recombinant human protein was used as the immunogen for the anti-Bcl6 antibody.

Immunogen: Recombinant human protein was used as the immunogen for the anti-Bcl6 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: B-cell lymphoma 6 protein

Background:

Target Description: Recognizes a protein of 95 kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Gene Symbol: BCL6///BCL5///LAZ3///ZBTB27///ZNF51

Gene ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus