CD44 antibody

Catalog No. ABIN6297335

The Mouse Monoclonal anti-CD44 antibody is suitable to detect CD44 in samples from Human and Non-Human Primate. It has been validated for FACS, IHC and IF.

Quick Overview for CD44 antibody (ABIN6297335)

Target: CD44

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD44 Antibody

Purpose: Mouse anti-Human/non-Human Primates CD44 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Recombinant human protein was used as the immunogen for the CD44 antibody.

Immunogen: Recombinant human protein was used as the immunogen for the CD44 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD44

Target: CD44

Alternative Name: CD44 antigen

Background:

Target Description: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. It is a receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. [UniProt]

Gene Symbol: CD44///LHR///MDU2///MDU3///MIC4

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling