Nucleolin antibody

Catalog No. ABIN6297424

This Mouse Monoclonal antibody specifically detects Nucleolin in WB, IHC, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Nucleolin antibody (ABIN6297424)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Nucleolin Antibody

Purpose: Mouse anti-Human/Mouse Nucleolin Antibody [Sodium Azide Free]

Specificity: Nucleoli

Purification: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Application Details

Application Notes: IF: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Western blot: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background:

Target Description: Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Gene Symbol: NCL

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization