Nucleolin antibody

Catalog No. ABIN6297426

This anti-Nucleolin antibody is a Mouse Monoclonal antibody detecting Nucleolin in WB, IHC, IF and FACS. Suitable for Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat and Gerbil.

Quick Overview for Nucleolin antibody (ABIN6297426)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat, Gerbil

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Nucleolin Antibody

Purpose: Mouse anti-Human/Mouse/Rat/Guinea Pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken Nucleolin Antibody [Sodium Azide Free]

Specificity: Nucleoli

Purification: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Immunogen: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
IF: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Western blot: 0.5-1 μg/mL
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background:

Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Gene Symbol: NCL

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization