TYR antibody

Catalog No. ABIN6297626

The Mouse Monoclonal anti-TYR antibody has been validated for IHC, IF and FACS. It is suitable to detect TYR in samples from Human.

Quick Overview for TYR antibody (ABIN6297626)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TYR Antibody

Purpose: Mouse anti-Human Tyrosinase Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Recombinant tyrosinase protein was used as the immunogen for this Tyrosinase antibody.

Immunogen: Recombinant tyrosinase protein was used as the immunogen for this Tyrosinase antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: Tyrosinase

Background:

Target Description: Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.

Gene Symbol: TYR

Gene ID: 7299

UniProt: P14679