Keratin 10 antibody

Catalog No. ABIN6297653

The Mouse Monoclonal anti-Keratin 10 antibody has been validated for IHC, WB, FACS and IF. It is suitable to detect Keratin 10 in samples from Human, Mouse, Rat, Dog, Rabbit, Monkey, Cow, Chicken and Turtle.

Quick Overview for Keratin 10 antibody (ABIN6297653)

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Monkey, Cow, Chicken, Turtle

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Keratin 10 Antibody

Purpose: Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken/Turtle Acidic Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Solubilized keratin extract from human stratum corneum

Immunogen: Solubilized keratin extract from human stratum corneum

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Keratin, type I cytoskeletal 10

Background:

Target Description: Cytokeratins, also called keratins or simply CKs, are a family of fibrous structural proteins and a major structural component in the outer layer of human skin, as well as hair and nails.  There are 20 human epithelial keratins which can be divided into two subfamiles: acidic and basic (more recent human genone sequencing has identified an additional 20 members). The formation of cytokeratin intermediate filaments requires the pairing of at least one acidic and one basic subfamily member. Members of the same keratin subfamily share extended sequence homology while members of different subfamilies show only limited sequence homology.This antibody is specific for the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), and 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

Gene Symbol: KRT10///KPP

Gene ID: 3858

UniProt: P13645