Cytokeratin 1 antibody

Catalog No. ABIN6297656

The Mouse Monoclonal anti-Cytokeratin 1 antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect Cytokeratin 1 in samples from Human, Mouse and Rat.

Quick Overview for Cytokeratin 1 antibody (ABIN6297656)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Cytokeratin 1 Antibody

Purpose: Mouse anti-Human/Mouse/Rat HMW Cytokeratin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.

Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10e6 cells
IF: 0.5-1.0 μg/mL
WB: 0.5-1.0 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Keratin, type II cytoskeletal 1

Background:

Target Description: This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.

Gene Symbol: KRT1///KRTA

Gene ID: 3848

UniProt: P04264