GLG1 antibody

Catalog No. ABIN6297737

The Mouse Monoclonal anti-GLG1 antibody has been validated for WB, IHC, ICC, FACS and IF. It is suitable to detect GLG1 in samples from Human.

Quick Overview for GLG1 antibody (ABIN6297737)

Target: GLG1 (Golgi Glycoprotein 1 (GLG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-GLG1 Antibody

Purpose: Mouse anti-Human Golgi Marker Antibody [Sodium Azide Free]

Specificity: Golgi complex in cytoplasm

Purification: The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.

Immunogen: The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
Western blot: 0.5-1.0 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for GLG1

Target: GLG1 (Golgi Glycoprotein 1 (GLG1))

Alternative Name: Golgi apparatus protein 1

Background:

Target Description: This mAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This mAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Gene Symbol: GLG1///CFR1///ESL1///MG160

Gene ID: 2734

UniProt: Q92896