Mitochondrial Marker antibody

Catalog No. ABIN6297763

This Mouse Monoclonal antibody specifically detects in IF, WB, FACS and IHC. It exhibits reactivity toward Human.

Quick Overview for Mitochondrial Marker antibody (ABIN6297763)

Target: Mitochondrial Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details

Purpose: Mouse anti-Human Mitochondria Marker Antibody [Sodium Azide Free]

Specificity: Mitochondria in cytoplasm

Purification: The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.

Immunogen: The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Mitochondrial Marker

Alternative Name: Mitochondria Marker

Background:

Target Description: MAb MTC754 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.