CD63 antibody

Catalog No. ABIN6297773

The Mouse Monoclonal anti-CD63 antibody has been validated for FACS, WB, IHC and IF. It is suitable to detect CD63 in samples from Human and Mouse.

Quick Overview for CD63 antibody (ABIN6297773)

Target: CD63

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD63 Antibody

Purpose: Mouse anti-Human/Mouse CD63 Antibody [Sodium Azide Free]

Specificity: Cell Surface & Cytoplasmic

Purification: The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this anti-CD63 antibody.

Immunogen: The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this anti-CD63 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 2-4 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD63

Target: CD63

Alternative Name: CD63 antigen

Background:

Target Description: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Gene Symbol: CD63///MLA1///TSPAN30

Gene ID: 967

UniProt: P08962