MecA PBP2 antibody (Biotin)

Catalog No. ABIN6297798

This anti-MecA PBP2 antibody is a Mouse Monoclonal antibody detecting MecA PBP2 in ELISA and WB. Suitable for Human.

Quick Overview for MecA PBP2 antibody (Biotin) (ABIN6297798)

Target: MecA PBP2 (Penicillin Binding Protein 2a (MecA PBP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MecA PBP2 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Clone: 5B9-G2-F12

Product Details anti-MecA PBP2 Antibody (Biotin)

Purpose: Mouse anti-Human PBP2a Biotinylated Antibody

Immunogen: This antibody was produced by fusion of mouse myeloma with B cells obtained from a mouse immunized with a recombinant protein immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.

Application Details

Application Notes: ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/mL (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l

Restrictions: For Research Use only

Handling

Reconstitution: A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e. g. sodium azide). The final amount is indicated on the shipping vial.

Storage: 4 °C,-20 °C

Storage Comment: -20°C

Target Details for MecA PBP2

Target: MecA PBP2 (Penicillin Binding Protein 2a (MecA PBP2))

Alternative Name: PBP2a

Background:

Target Description: PBP2A has a low affinity for beta-lactam antibiotics such as methicillin and penicillin. This enables transpeptidase activity in the presence of beta-lactams, preventing them from inhibiting cell wall synthesis.

UniProt: Q53707