Nucleolin antibody (C-Term)

Catalog No. ABIN629901

This anti-Nucleolin antibody is a Rabbit Polyclonal antibody detecting Nucleolin in WB and IHC. Suitable for Human, Rat and Dog.

Quick Overview for Nucleolin antibody (C-Term) (ABIN629901)

Target: Nucleolin (NCL)

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Nucleolin Antibody

Binding Specificity: C-Term

Specificity: Nucleolin antibody was raised against the C terminal of NCL

Purification: Purified

Immunogen: Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

Application Details

Application Notes: WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Nucleolin Blocking Peptide, (ABIN938664), is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NCL antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Molecular Weight: 78 kDa (MW of target protein)

Pathways: Ribonucleoprotein Complex Subunit Organization