Erythrocyte Ankyrin antibody (C-Term)

Catalog No. ABIN630429

Product Details anti-Erythrocyte Ankyrin Antibody

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Erythrocyte Ankyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Ankyrin 1 antibody was raised against the C terminal of ANK1

Purification: Purified

Immunogen: Ankyrin 1 antibody was raised using the C terminal of ANK1 corresponding to a region with amino acids VVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Application Details

Application Notes: WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Ankyrin 1 Blocking Peptide, catalog no. 33R-9893, is also available for use as a blocking control in assays to test for specificity of this Ankyrin 1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of ANK1 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: Ankyrin 1 (ANK1 Products)

Background: Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.

Molecular Weight: 17 kDa (MW of target protein)

Pathways: Synaptic Membrane