MBD1 antibody (Middle Region)

Catalog No. ABIN630596

Product Details anti-MBD1 Antibody

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: MBD1 antibody was raised against the middle region of MBD1

Purification: Affinity purified

Immunogen: MBD1 antibody was raised using the middle region of MBD1 corresponding to a region with amino acids CKVWETEDTVEPTSTSWNPRGWPGTHVSLSPPPASMMWVSCRRSWCPSSQ

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

MBD1 Blocking Peptide, catalog no. 33R-1727, is also available for use as a blocking control in assays to test for specificity of this MBD1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of MBD1 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1 (MBD1 Products)

Synonyms: CXXC3 antibody, PCM1 antibody, RFT antibody, Cxxc3 antibody, CCDC11 antibody, methyl-CpG binding domain protein 1 antibody, MBD1 antibody, Mbd1 antibody

Background: MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus.

Molecular Weight: 65 kDa (MW of target protein)