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GAPDH antibody (N-Term)

Name: GAPDH antibody (N-Term)
Brand: antibodies-online
SKU: ABIN630723
Price: 1406.78 USD
Availability: InStock

GAPDH Reactivity: Human, Dog WB Host: Rabbit Polyclonal unconjugated

GAPDH antibody used at 1 ug/ml to detect target protein. (GAPDH antibody (N-Term))

1 image

(2 references)

Catalog No. ABIN630723

Datasheet as PDF

Target See all GAPDH Antibodies

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Binding Specificity
34

19

13

12

7

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
209

144

127

63

46

39

25

24

23

19

19

19

17

15

14

14

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
Human, Dog
Host
160

83

16

11

4
Rabbit
Compatible Secondaries
Clonality
183

89
Polyclonal
Conjugate
153

31

18

16

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
Application
218

101

72

65

38

34

30

26

16

15

15

15

7

6

5

4

3

3

1

1

1

1

1

1
Western Blotting (WB)

Specificity

GAPDH antibody was raised against the N terminal of GAPDH

Purification

Affinity purified

Immunogen

GAPDH antibody was raised using the N terminal of GAPDH corresponding to a region with amino acids IDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKW

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Verified GAPDH Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment

GAPDH Blocking Peptide, catalog no. 33R-3921, is also available for use as a blocking control in assays to test for specificity of this GAPDH antibody

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Lyophilized powder. Add distilled water for a 1 mg/mL concentration of GAPDH antibody in PBS

Concentration

Lot specific

Buffer

PBS

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
Kuroda, Ishii, Uematsu, Ohata, Coban, Akira, Aritake, Urade, Morimoto: "Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms." in: Immunity, Vol. 34, Issue 4, pp. 514-26, (2011) (PubMed).

Shi, Zhang, Yang, Zhang, Wei: "ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice." in: Journal of molecular and cellular cardiology, Vol. 49, Issue 5, pp. 819-28, (2010) (PubMed).
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name

GAPDH (GAPDH Products)

Background

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

Molecular Weight

36 kDa (MW of target protein)