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ALAD antibody (N-Term)

This Rabbit Polyclonal antibody specifically detects ALAD in WB. It exhibits reactivity toward Human.
Catalog No. ABIN632017

Quick Overview for ALAD antibody (N-Term) (ABIN632017)

Target

See all ALAD Antibodies
ALAD (Aminolevulinate Dehydratase (ALAD))

Reactivity

  • 26
  • 20
  • 9
  • 6
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  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 39
  • 7
Rabbit

Clonality

  • 42
  • 4
Polyclonal

Conjugate

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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ALAD antibody is un-conjugated

Application

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  • 2
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
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    • 1
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    N-Term

    Specificity

    ALAD antibody was raised against the N terminal of ALAD

    Purification

    Affinity purified

    Immunogen

    ALAD antibody was raised using the N terminal of ALAD corresponding to a region with amino acids QPQSVLHSGYFHPLLRAWQTATTTLNASNLIYPIFVTDVPDDIQPITSLP
  • Application Notes

    WB: 1 µg/mL
    Optimal conditions should be determined by the investigator.

    Comment

    ALAD Blocking Peptide, (ABIN5612001), is also available for use as a blocking control in assays to test for specificity of this ALAD antibody

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Lyophilized powder. Add distilled water for a 1 mg/mL concentration of ALAD antibody in PBS

    Concentration

    Lot specific

    Buffer

    PBS

    Handling Advice

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
  • Target

    ALAD (Aminolevulinate Dehydratase (ALAD))

    Alternative Name

    ALAD

    Background

    The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.

    Molecular Weight

    37 kDa (MW of target protein)
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