CA5B antibody (N-Term)

Catalog No. ABIN633032

The Rabbit Polyclonal anti-CA5B antibody has been validated for WB. It is suitable to detect CA5B in samples from Human.

Quick Overview for CA5B antibody (N-Term) (ABIN633032)

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA5B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CA5B Antibody

Binding Specificity: N-Term

Specificity: Carbonic Anhydrase Vb antibody (Mitochondrial) was raised against the N terminal of CA5 B

Purification: Affinity purified

Immunogen: Carbonic Anhydrase Vb antibody (Mitochondrial) was raised using the N terminal of CA5 B corresponding to a region with amino acids WRDSVYDPGLKPLTISYDPATCLHVWNNGYSFLVEFEDSTDKSVIKGGPL

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Carbonic Anhydrase Vb Blocking Peptide (Mitochondrial), (ABIN5612606), is also available for use as a blocking control in assays to test for specificity of this Carbonic Anhydrase Vb antibody (Mitochondrial)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of CA0 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for CA5B

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: Carbonic Anhydrase Vb

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.

Molecular Weight: 33 kDa (MW of target protein)