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Nucleolin antibody (N-Term)

NCL Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN633315

Datasheet as PDF

Target See all Nucleolin (NCL) Antibodies

Nucleolin (NCL)
Binding Specificity
15

9

8

7

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
111

31

28

10

10

9

9

8

6

4

4

4

2

1

1
Human
Host
81

35

1
Rabbit
Compatible Secondaries
Clonality
73

44
Polyclonal
Conjugate
68

8

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Nucleolin antibody is un-conjugated
Application
89

53

52

43

29

19

15

14

14

13

12

10

3

3

2

1
Western Blotting (WB), Immunohistochemistry (IHC)

Specificity

Nucleolin antibody was raised against the N terminal of NCL

Purification

Affinity purified

Immunogen

Nucleolin antibody was raised using the N terminal of NCL corresponding to a region with amino acids GKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDED

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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Nucleolin (NCL) antibody
NCL Reactivity: Human WB, IHC, IF, FACS, ISt, StM Host: Mouse Monoclonal NCL-902 unconjugated

anti-Nucleolin (NCL) (AA 388-569) antibody
NCL Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Nucleolin (NCL) antibody
NCL Reactivity: Human WB, IHC, IF, FACS, ISt Host: Mouse Monoclonal NCL-902 unconjugated

anti-Nucleolin (NCL) antibody
NCL Reactivity: Human WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated

anti-Nucleolin (NCL) antibody
NCL Reactivity: Human WB, IF, FACS, IHC (p) Host: Mouse Monoclonal 364-5-NCL-902 unconjugated

anti-Nucleolin (NCL) antibody
NCL Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat, Gerbil WB, IF, FACS, IHC (p) Host: Mouse Monoclonal NCL-902 unconjugated

anti-Nucleolin (NCL) antibody
NCL Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Guinea Pig, Chicken, Cat, Gerbil WB, IF, FACS, IHC (p) Host: Mouse Monoclonal NCL-902 unconjugated

anti-Nucleolin (NCL) (AA 428-455) antibody
NCL Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17464 unconjugated

anti-Nucleolin (NCL) (C-Term) antibody
NCL Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 0.5 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment

Nucleolin Blocking Peptide, catalog no. 33R-3357, is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NCL antibody in PBS

Concentration

Lot specific

Buffer

PBS

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
Target

Nucleolin (NCL)

Alternative Name

Nucleolin (NCL Products)

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb.

Molecular Weight

78 kDa (MW of target protein)

Pathways

Ribonucleoprotein Complex Subunit Organization