PABPC4 antibody (Middle Region)

Catalog No. ABIN633447

The Rabbit Polyclonal anti-PABPC4 antibody (ABIN633447) specifically detects PABPC4 in WB. The antibody is reactive with Human samples.

Quick Overview for PABPC4 antibody (Middle Region) (ABIN633447)

Target: PABPC4 (Poly(A) Binding Protein, Cytoplasmic 4 (Inducible Form) (PABPC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PABPC4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PABPC4 Antibody

Binding Specificity: Middle Region

Specificity: PABPC4 antibody was raised against the middle region of PABPC4

Purification: Affinity purified

Immunogen: PABPC4 antibody was raised using the middle region of PABPC4 corresponding to a region with amino acids RPNPRWQQGGRPQGFQGMPSAIRQSGPRPTLRHLAPTGSECPDRLAMDFG

Application Details

Application Notes: WB: 0.5 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

PABPC4 Blocking Peptide, (ABIN5615185), is also available for use as a blocking control in assays to test for specificity of this PABPC4 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of PABPC4 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for PABPC4

Target: PABPC4 (Poly(A) Binding Protein, Cytoplasmic 4 (Inducible Form) (PABPC4))

Alternative Name: PABPC4

Background: Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets.

Molecular Weight: 71 kDa (MW of target protein)

Pathways: SARS-CoV-2 Protein Interactome