SPPL2B antibody (N-Term)

Catalog No. ABIN634985

The Rabbit Polyclonal anti-SPPL2B antibody has been validated for WB and IHC. It is suitable to detect SPPL2B in samples from Human.

Quick Overview for SPPL2B antibody (N-Term) (ABIN634985)

Target: SPPL2B (Signal Peptide Peptidase-Like 2B (SPPL2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPPL2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPPL2B Antibody

Binding Specificity: N-Term

Specificity: SPPL2 B antibody was raised against the N terminal of SPPL2

Purification: Affinity purified

Immunogen: SPPL2 B antibody was raised using the N terminal of SPPL2 corresponding to a region with amino acids VARGNCTFYEKVRLAQGSGARGLLIVSRERLVPPGGNKTQYDEIGIPVAL

Application Details

Application Notes: WB: 1 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

SPPL2B Blocking Peptide, (ABIN5616378), is also available for use as a blocking control in assays to test for specificity of this SPPL2B antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of SPPL0 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for SPPL2B

Target: SPPL2B (Signal Peptide Peptidase-Like 2B (SPPL2B))

Alternative Name: SPPL2B

Background: SPPL2B is a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways.

Molecular Weight: 56 kDa (MW of target protein)