Nestin antibody (AA 254-270)

Catalog No. ABIN636323

The Rabbit Polyclonal anti-Nestin antibody has been validated for WB, IHC and FACS. It is suitable to detect Nestin in samples from Human.

Quick Overview for Nestin antibody (AA 254-270) (ABIN636323)

Target: Nestin (NES)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Nestin Antibody

Binding Specificity: AA 254-270

Cross-Reactivity: Mouse (Murine)

Purification: Affinity chromatography purified

Immunogen: Nestin antibody was raised in rabbit using AA 254-270 [RATEKFQLAVEALEQEK] of the 177 kDa human nestin protein as the immunogen.

Isotype: IgG

Application Details

Application Notes: FC: 1:200-1:500, IHC: 1:100-1:200, WB: 1:500-1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Affinity purified and supplied in PBS, with 0.02 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin is an intermediate filament protein that is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon differentiation, Nestin is replaced by cell type-specific intermediate filaments, (e.g., neurofilaments and glial fibrillary acidic protein (GFAP)). Nestin expression is re-induced after CNS injury and during regeneration of injured muscle tissue.