Dinitrophenol antibody

Catalog No. ABIN636762

Cited in 1 publication. The Goat Polyclonal anti-Dinitrophenol antibody (ABIN636762) specifically detects Dinitrophenol in ELISA and WB.

Quick Overview for Dinitrophenol antibody (ABIN636762)

Target: Dinitrophenol (DNP)

Host: Goat

Clonality: Polyclonal

Conjugate: This Dinitrophenol antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-Dinitrophenol Antibody

Immunogen: Dinitrophenol antibody was raised in goat using dinitrophenol-modified protein as the immunogen.

Application Details

Application Notes: ELISA: >1:4,000, WB: >1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as liquid whole serum without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References for anti-Dinitrophenol antibody (ABIN636762)

Fan, Schrott, Snelling, Felty, Graham, McGauly, Arnold, Korneeva: "Carbonyl-protein content increases in brain and blood of female rats after chronic oxycodone treatment." in: BMC neuroscience, Vol. 21, Issue 1, pp. 4, (2020) (PubMed).

Target Details for Dinitrophenol

Target: Dinitrophenol (DNP)

Alternative Name: DNP

Target Type: Chemical

Background: Tissues are continually exposed to reactive oxygen species (ROS) that are produced in tissues from metabolism processes or the inflammatory response of leukocytes or macrophages. It is estimated that as much as 1 % of consumed oxygen may be converted to ROS, which can cause damage to various cellular components. Proteins are one of the cellular components most vulnerable to oxidative damage by ROS, which results in an increase in protein carbonyl content. This oxidative modification of proteins can lead to cross-linking, peptide fragmentation, modified residues and the conversion of one amino group to another. If sufficient protein damage accumulates, cell death will occur.