SNCA antibody (AA 1-140)

Catalog No. ABIN364317

The Mouse Monoclonal anti-SNCA antibody (Clone 5C2) (ABIN364317) specifically detects SNCA in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for SNCA antibody (AA 1-140) (ABIN364317)

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C2

Product Details anti-SNCA Antibody

Binding Specificity: AA 1-140

Specificity: NAC domain of alpha-synuclein (aa 61-95)

Purification: Protein G purified

Immunogen: Recombinant human kappa-synuclein (1-140) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: SNCA / alpha-Synuclein

Background: Name/Gene ID: SNCA

Synonyms: SNCA, Alpha-synuclein, PARK4, PD1, Synuclein alpha-140, NACP, PARK1

Gene ID: 6622

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity