AFT1 antibody (N-Term)

Catalog No. ABIN6391338

The Goat Polyclonal anti-AFT1 antibody (ABIN6391338) specifically detects AFT1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for AFT1 antibody (N-Term) (ABIN6391338)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-AFT1 Antibody

Binding Specificity: N-Term

Purpose: ATF1

Sequence: EDSHKSTTSE TAPQ

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in nuclear lysates of cell line A431 (calculated MW of 29.2 kDa according to NP_005162.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: ATF1, activating transcription factor 1, EWS-ATF1, FUS/ATF-1, TREB36, cAMP-dependent transcription factor ATF-1, cyclic AMP-dependent transcription factor ATF-1

Gene ID: 466

NCBI Accession: NP_005162

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades