BRD4 antibody (Internal Region)

Catalog No. ABIN6391339

This Goat Polyclonal antibody specifically detects BRD4 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for BRD4 antibody (Internal Region) (ABIN6391339)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BRD4 Antibody

Binding Specificity: Internal Region

Purpose: BRD4

Sequence: PETSNPNKPK RQTNQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_490597.1, NP_055114.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in Mouse Testis lysates (calculated MW of 155.8 kDa according to Mouse NP_065254.3). Recommended concentration: 0.5 -2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background: BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, bromodomain-containing 4, bromodomain-containing protein 4, chromosome-associated protein

Gene ID: 23476

NCBI Accession: NP_490597, NP_055114

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome