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MCAM antibody (Internal Region)

The Goat Polyclonal anti-MCAM antibody has been validated for WB and ELISA. It is suitable to detect MCAM in samples from Human.
Catalog No. ABIN6391340

Quick Overview for MCAM antibody (Internal Region) (ABIN6391340)

Target

See all MCAM Antibodies
MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity

  • 174
  • 55
  • 54
  • 15
  • 15
  • 8
  • 8
  • 6
  • 5
  • 2
Human

Host

  • 108
  • 102
  • 2
  • 1
  • 1
Goat

Clonality

  • 116
  • 98
Polyclonal

Conjugate

  • 97
  • 17
  • 14
  • 9
  • 8
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MCAM antibody is un-conjugated

Application

  • 111
  • 97
  • 79
  • 41
  • 33
  • 30
  • 28
  • 24
  • 12
  • 12
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA

Grade

Verified
  • Binding Specificity

    • 24
    • 16
    • 9
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Purpose

    MCAM / CD146/ MUC18

    Sequence

    EPARKEHSGR

    Cross-Reactivity

    Human

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 70-75 kDa band observed in Human Heart, Placenta and Umbilical Cord lysates (calculated MWt of 71.5 kDa according to NP_006491.2) Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    MCAM (Melanoma Cell Adhesion Molecule (MCAM))

    Alternative Name

    MCAM

    Background

    MCAM, melanoma cell adhesion molecule, CD146, MUC18, Gicerin, S-endo 1 endothelial-associated antigen, cell surface glycoprotein MUC18, cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma-associated antigen A32, melanoma-associated antig

    Gene ID

    4162

    NCBI Accession

    NP_006491
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