Doublecortin antibody (AA 69-81)

Catalog No. ABIN6391364

The Goat Polyclonal anti-Doublecortin antibody has been validated for ELISA, IHC, IF and FACS. It is suitable to detect Doublecortin in samples from Human.

Quick Overview for Doublecortin antibody (AA 69-81) (ABIN6391364)

Target: Doublecortin (DCX)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Doublecortin Antibody

Binding Specificity: AA 69-81

Purpose: Doublecortin / DCX (aa69-81)

Sequence: DRSHCQSLRF HQN

Specificity: This antibody is expected to recognize reported isoform a (NP_000546.2) only.

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/m

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX

Background: DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin

Gene ID: 1641

NCBI Accession: NP_000546