NEFL antibody (AA 330-343)

Catalog No. ABIN6391366

This Goat Polyclonal antibody specifically detects NEFL in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NEFL antibody (AA 330-343) (ABIN6391366)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NEFL Antibody

Binding Specificity: AA 330-343

Purpose: NEFL (aa330-343)

Sequence: EKQLQELEDK QNAD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 68-70 kDa band observed in lysates of Neuroblastoma cell line KELLY (calculated MW of 61.5 kDa according to NP_006149.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NEFL

Background: NEFL, neurofilament, light polypeptide, CMT1F, CMT2E, NF-L, NF68, NFL, PPP1R110, light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptid

Gene ID: 4747, 18039, 83613

NCBI Accession: NP_006149