LAG3 antibody (Internal Region)

Catalog No. ABIN6391370

The Goat Polyclonal anti-LAG3 antibody has been validated for WB and ELISA. It is suitable to detect LAG3 in samples from Human.

Quick Overview for LAG3 antibody (Internal Region) (ABIN6391370)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LAG3 Antibody

Binding Specificity: Internal Region

Purpose: LAG3 / CD223

Sequence: EDVSQAQAGT YTCH

Specificity: This antibody is expected to recognize the extra-cellular domain.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 57.4 kDa according to NP_002277.4). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3

Background: LAG3, lymphocyte activating 3, CD223, lymphocyte-activation gene 3

Gene ID: 3902

NCBI Accession: NP_002277

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints