PDGFB antibody (C-Term)

Catalog No. ABIN6391413

The Goat Polyclonal anti-PDGFB antibody (ABIN6391413) specifically detects PDGFB in ELISA, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for PDGFB antibody (C-Term) (ABIN6391413)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDGFB antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PDGFB Antibody

Binding Specificity: C-Term

Purpose: PDGFB precursor

Sequence: KHRKFKHTHD KT

Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_002599.1, NP_148937.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the vesicles of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGFB

Background: PDGFB, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), FLJ12858, PDGF2, SIS, SSV, c-sis, PDGF, B chain, Platelet-derived growth factor, beta polypeptide (oncogene SIS), becaplermin, oncogene SIS, platelet-d

Gene ID: 5155, 18591, 24628

NCBI Accession: NP_002599, NP_148937

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling