MECP2 antibody (pSer423)

Catalog No. ABIN650833

This Rabbit Polyclonal antibody specifically detects MECP2 in WB and IHC (p). It exhibits reactivity toward Human and Rat and has been mentioned in 2+ publications.

Quick Overview for MECP2 antibody (pSer423) (ABIN650833)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB29261

Product Details anti-MECP2 Antibody

Binding Specificity: pSer423

Predicted Reactivity: Pr, M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S423 of human MeCP2.

Isotype: IgG

Application Details

Application Notes: WB: 1:500. WB: 1:500. WB: 1:500. IHC-P-Leica: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-MECP2 antibody (ABIN650833)

Qiu, Sylwestrak, Lieberman, Zhang, Liu, Ghosh: "The Rett syndrome protein MeCP2 regulates synaptic scaling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 3, pp. 989-94, (2012) (PubMed).

Stansfield, Pilsner, Lu, Wright, Guilarte: "Dysregulation of BDNF-TrkB signaling in developing hippocampal neurons by Pb(2+): implications for an environmental basis of neurodevelopmental disorders." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 127, Issue 1, pp. 277-95, (2012) (PubMed).

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Molecular Weight: 52441

Gene ID: 4204

NCBI Accession: NP_001104262, NP_004983

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane