WNT5A antibody (AA 185-213)

Catalog No. ABIN651869

The Rabbit Polyclonal anti-WNT5A antibody has been validated for WB, FACS and IHC (p). It is suitable to detect WNT5A in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for WNT5A antibody (AA 185-213) (ABIN651869)

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT5A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB22340

Product Details anti-WNT5A Antibody

Binding Specificity: AA 185-213

Predicted Reactivity: Rb

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human WNT5A.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:500. IHC-P: 1:50~100. IHC-P-Leica: 1:100. FC: 1:10~50. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-WNT5A antibody (ABIN651869)

Wu, Xiong, Wang, Miao, Liu, Li, Zou, Liu, Zhao, Yang: "Comparative study of ROR2 and WNT5a expression in squamous/adenosquamous carcinoma and adenocarcinoma of the gallbladder." in: World journal of gastroenterology, Vol. 23, Issue 14, pp. 2601-2612, (2018) (PubMed).

Target Details for WNT5A

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: WNT5A

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98 % , 98 % and 87 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].

Molecular Weight: 42339

Gene ID: 7474

NCBI Accession: NP_001243034, NP_003383

UniProt: P41221

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation