WNT5A antibody (AA 180-229)

Catalog No. ABIN574490

The Rabbit Polyclonal anti-WNT5A antibody is suitable to detect WNT5A in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Bat and Pig. It has been validated for WB, IHC and IHC (p).

Quick Overview for WNT5A antibody (AA 180-229) (ABIN574490)

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WNT5A Antibody

Binding Specificity: AA 180-229

Specificity: Human WNT5A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig (100%) Chicken, Xenopus (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human WNT5A (P41221, NP_003383). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig, Platypus, Lizard (100%), Monkey, Zebra finch, Chicken, Xenopus (93%), Orangutan, Hamster, Horse, Turkey (86%), Panda (81%), Stickleback, Zebrafish (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for WNT5A

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: WNT5A

Background: Name/Gene ID: WNT5A
Family: WNT

Synonyms: WNT5A, HWNT5A, Protein Wnt-5a, WNT-5A protein

Gene ID: 7474

NCBI Accession: NP_003383

UniProt: P41221

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation